Journal: Applied and Environmental Microbiology

Article Title: Methanogenesis from Methanol at Low Temperatures by a Novel Psychrophilic Methanogen, “ Methanolobus psychrophilus ” sp. nov., Prevalent in Zoige Wetland of the Tibetan Plateau

doi: 10.1128/aem.01146-08

Figure Lengend Snippet: FIG. 1. Electron micrographs of Methanolobus psychrophilus R15 growing in 20 mM methanol at 18°C. (A) Cell with two polar flagella; (B) cell aggregate. Bars 0.5 m.

Article Snippet: Differential characteristics that distinguish Methanolobus psychrophilus R15 from described Methanolobus species Characteristic Description or value M. psychrophilus R15T M. tindarius ATCC 35996Ta M. oregonensis DSM 5435b M. taylorii DSM 9005c M. vulcani DSM 3029d M. bombayensis DSM 7082e Morphology Coccoid cells Loose aggregates Flagella One or two polar Monotrichous Substrate Methyl sulfides Growth factor requirement Biotin NRf Biotin, thiamine Biotin Biotin Growth Temp range/optimal temp (°C) 0–25/18 7–50/28 7–44/34 11–40/39 13–45/40 13–46/37 pH 6.0–8.0 5.5–8.0 7.4–9.5 7.0–8.6 5.5–8.0 6.0–8.5 NaCl (mM) 10–800 60–1,270 100–1,600 200–1,200 100–1,200 300–2,000 Methanol conversion rate (mM h 1) 0°C 0.016 0 0g 0g 0g 0g 18°C 0.36 NRf 0.13g 0.099g 0.048g 0.057g 25°C 0.001 NRf 0.32g 0.24g 0.27g 0.37g Mol% G C of DNA 44.9 46 41 41 39 39 a Data are from reference 20. b Data are from reference 25. c Data are from reference 28. d Data are from reference 18. e Data are from reference 19. f NR, not reported. g This study. on M ay 30, 2015 by N D S U http://aem .asm .org/ D ow nloaded from rRNA primers, the 16S rRNA gene copies of strain R15 and total archaea were determined to be 5.5 107 and 3.2 108 per gram of soil, respectively (Table 3).

Techniques:

Journal: Applied and Environmental Microbiology

Article Title: Methanogenesis from Methanol at Low Temperatures by a Novel Psychrophilic Methanogen, “ Methanolobus psychrophilus ” sp. nov., Prevalent in Zoige Wetland of the Tibetan Plateau

doi: 10.1128/aem.01146-08

Figure Lengend Snippet: FIG. 2. Growth of Methanolobus psychrophilus R15 on 20 mM methanol. (A) Specific growth rates at various temperatures. Data are means for three replicates. (B) Curves of log OD550 (open circles) and methane production (filled circles) at 18°C. Data are means for three replicates. Standard deviations are shown.

Article Snippet: Differential characteristics that distinguish Methanolobus psychrophilus R15 from described Methanolobus species Characteristic Description or value M. psychrophilus R15T M. tindarius ATCC 35996Ta M. oregonensis DSM 5435b M. taylorii DSM 9005c M. vulcani DSM 3029d M. bombayensis DSM 7082e Morphology Coccoid cells Loose aggregates Flagella One or two polar Monotrichous Substrate Methyl sulfides Growth factor requirement Biotin NRf Biotin, thiamine Biotin Biotin Growth Temp range/optimal temp (°C) 0–25/18 7–50/28 7–44/34 11–40/39 13–45/40 13–46/37 pH 6.0–8.0 5.5–8.0 7.4–9.5 7.0–8.6 5.5–8.0 6.0–8.5 NaCl (mM) 10–800 60–1,270 100–1,600 200–1,200 100–1,200 300–2,000 Methanol conversion rate (mM h 1) 0°C 0.016 0 0g 0g 0g 0g 18°C 0.36 NRf 0.13g 0.099g 0.048g 0.057g 25°C 0.001 NRf 0.32g 0.24g 0.27g 0.37g Mol% G C of DNA 44.9 46 41 41 39 39 a Data are from reference 20. b Data are from reference 25. c Data are from reference 28. d Data are from reference 18. e Data are from reference 19. f NR, not reported. g This study. on M ay 30, 2015 by N D S U http://aem .asm .org/ D ow nloaded from rRNA primers, the 16S rRNA gene copies of strain R15 and total archaea were determined to be 5.5 107 and 3.2 108 per gram of soil, respectively (Table 3).

Techniques:

Journal: Applied and Environmental Microbiology

Article Title: Methanogenesis from Methanol at Low Temperatures by a Novel Psychrophilic Methanogen, “ Methanolobus psychrophilus ” sp. nov., Prevalent in Zoige Wetland of the Tibetan Plateau

doi: 10.1128/aem.01146-08

Figure Lengend Snippet: FIG. 3. Phylogenetic tree showing the position of strain R15 among other species of the genus Methanolobus. Based on a consensus 1,378 bp of the 16S rRNA gene, the tree was constructed by the neighbor- joining method. The topology of the tree was estimated by bootstraps based on 1,000 replications. The number on each branch node is the percentage supported by bootstraps. GenBank accession numbers of 16S rRNA sequences are given in parentheses. Bar, 1% sequence divergence.

Article Snippet: Differential characteristics that distinguish Methanolobus psychrophilus R15 from described Methanolobus species Characteristic Description or value M. psychrophilus R15T M. tindarius ATCC 35996Ta M. oregonensis DSM 5435b M. taylorii DSM 9005c M. vulcani DSM 3029d M. bombayensis DSM 7082e Morphology Coccoid cells Loose aggregates Flagella One or two polar Monotrichous Substrate Methyl sulfides Growth factor requirement Biotin NRf Biotin, thiamine Biotin Biotin Growth Temp range/optimal temp (°C) 0–25/18 7–50/28 7–44/34 11–40/39 13–45/40 13–46/37 pH 6.0–8.0 5.5–8.0 7.4–9.5 7.0–8.6 5.5–8.0 6.0–8.5 NaCl (mM) 10–800 60–1,270 100–1,600 200–1,200 100–1,200 300–2,000 Methanol conversion rate (mM h 1) 0°C 0.016 0 0g 0g 0g 0g 18°C 0.36 NRf 0.13g 0.099g 0.048g 0.057g 25°C 0.001 NRf 0.32g 0.24g 0.27g 0.37g Mol% G C of DNA 44.9 46 41 41 39 39 a Data are from reference 20. b Data are from reference 25. c Data are from reference 28. d Data are from reference 18. e Data are from reference 19. f NR, not reported. g This study. on M ay 30, 2015 by N D S U http://aem .asm .org/ D ow nloaded from rRNA primers, the 16S rRNA gene copies of strain R15 and total archaea were determined to be 5.5 107 and 3.2 108 per gram of soil, respectively (Table 3).

Techniques: Construct, Sequencing

Journal: Applied and Environmental Microbiology

Article Title: Methanogenesis from Methanol at Low Temperatures by a Novel Psychrophilic Methanogen, “ Methanolobus psychrophilus ” sp. nov., Prevalent in Zoige Wetland of the Tibetan Plateau

doi: 10.1128/aem.01146-08

Figure Lengend Snippet: FIG. 4. Methanol degradation (open circles) and methane produc- tion (filled circles) by Methanolobus psychrophilus R15 growing cells at a methanol concentration of 20 mM, incubated at 18°C (A) and 0°C (B). Data are means for three replicates. Standard deviations are shown.

Article Snippet: Differential characteristics that distinguish Methanolobus psychrophilus R15 from described Methanolobus species Characteristic Description or value M. psychrophilus R15T M. tindarius ATCC 35996Ta M. oregonensis DSM 5435b M. taylorii DSM 9005c M. vulcani DSM 3029d M. bombayensis DSM 7082e Morphology Coccoid cells Loose aggregates Flagella One or two polar Monotrichous Substrate Methyl sulfides Growth factor requirement Biotin NRf Biotin, thiamine Biotin Biotin Growth Temp range/optimal temp (°C) 0–25/18 7–50/28 7–44/34 11–40/39 13–45/40 13–46/37 pH 6.0–8.0 5.5–8.0 7.4–9.5 7.0–8.6 5.5–8.0 6.0–8.5 NaCl (mM) 10–800 60–1,270 100–1,600 200–1,200 100–1,200 300–2,000 Methanol conversion rate (mM h 1) 0°C 0.016 0 0g 0g 0g 0g 18°C 0.36 NRf 0.13g 0.099g 0.048g 0.057g 25°C 0.001 NRf 0.32g 0.24g 0.27g 0.37g Mol% G C of DNA 44.9 46 41 41 39 39 a Data are from reference 20. b Data are from reference 25. c Data are from reference 28. d Data are from reference 18. e Data are from reference 19. f NR, not reported. g This study. on M ay 30, 2015 by N D S U http://aem .asm .org/ D ow nloaded from rRNA primers, the 16S rRNA gene copies of strain R15 and total archaea were determined to be 5.5 107 and 3.2 108 per gram of soil, respectively (Table 3).

Techniques: Concentration Assay, Incubation

Journal: Environmental Microbiology

Article Title: Isolation and characterization of a novel methanogen Methanosarcina hadiensis sp. nov. from subsurface Boom Clay pore water

doi: 10.1111/1462-2920.70004

Figure Lengend Snippet: Phylogenetic tree created with Phylophlan 3.0 based on the provided dataset of 400 universal marker genes (Asnicar et al., ) illustrating the relationship of strain TD41E1‐1 (marked in blue) and other Methanosarcinaceae species.

Article Snippet: A 25 mL culture of an enrichment culture, strain TD41E1‐1, Methanolobus profundi MobM, Methanosarcina acetivorans C2A was incubated in liquid modified DSMZ 141c medium with 120 mM methanol and a 25 mL culture of Methanomassiliicoccus luminyensis B10 was incubated in liquid DSMZ 1637 medium.

Techniques: Marker

Journal: Environmental Microbiology

Article Title: Isolation and characterization of a novel methanogen Methanosarcina hadiensis sp. nov. from subsurface Boom Clay pore water

doi: 10.1111/1462-2920.70004

Figure Lengend Snippet: SEM pictures of pure cultures from M. acetivorans C2A (left), M. profundi MobM (middle) and M. luminiensis B10 (right).

Article Snippet: A 25 mL culture of an enrichment culture, strain TD41E1‐1, Methanolobus profundi MobM, Methanosarcina acetivorans C2A was incubated in liquid modified DSMZ 141c medium with 120 mM methanol and a 25 mL culture of Methanomassiliicoccus luminyensis B10 was incubated in liquid DSMZ 1637 medium.

Techniques: